Hotspot

psbC-trnS-UGA

LSC · 36001-36156 · 156 bp

Back to Schoenorchis

Pi 0.0839

MarkerSeek score 82.00

Primer available yes

Label psbC-trnS-UGA

Feature Metadata

label_name: psbC-trnS-UGA
feature_type: igs
region: LSC
length_bp: 156
variable_sites: 12
pi: 0.0839
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.92
markerseek_score: 82.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbC-trnS-UGA_p1	CCTGTTCTTTCCATGACCCC	GAGAGATGGCTGAGTGGTTG	55.0	55.0	58.2	58.0	265-278 bp	1.0	85.9
psbC-trnS-UGA_p2	CCTGTTCTTTCCATGACCCC	ATAGCCCCGGTCTTGAAAAC	55.0	50.0	58.2	57.9	245-258 bp	1.0	85.5
psbC-trnS-UGA_p3	TTCCATGACCCCTCTTAGCT	GAGAGATGGCTGAGTGGTTG	50.0	55.0	57.7	58.0	257-270 bp	1.0	85.3
psbC-trnS-UGA_p4	AGCGGGGTTTGAAAAAGGAA	GAGAGATGGCTGAGTGGTTG	45.0	55.0	58.2	58.0	302-315 bp	1.0	84.6
psbC-trnS-UGA_p5	TTGTGGGACATTTGTGGCAT	GAGAGATGGCTGAGTGGTTG	45.0	55.0	58.3	58.0	345-358 bp	1.0	83.2