Hotspot

trnG-GCC-trnR-UCU

LSC · 10260-10383 · 124 bp

Back to Schoenorchis

Pi 0.0887

MarkerSeek score 81.00

Primer available yes

Label trnG-GCC-trnR-UCU

Feature Metadata

label_name: trnG-GCC-trnR-UCU
feature_type: igs
region: LSC
length_bp: 124
variable_sites: 11
pi: 0.0887
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 81.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnG-GCC-trnR-UCU_p1	AGCCTTCCAAGCTAACGATG	AGAAGACCTTTGTCCTATCCA	50.0	42.9	58.0	55.7	204-205 bp	1.0	57.6
trnG-GCC-trnR-UCU_p2	CCTAGCCTTCCAAGCTAACG	AGAAGACCTTTGTCCTATCCA	55.0	42.9	58.1	55.7	207-208 bp	1.0	57.3
trnG-GCC-trnR-UCU_p3	AGCCTTCCAAGCTAACGATG	AGAAATTGCGTCCAATAGGA	50.0	40.0	58.0	54.7	246-247 bp	1.0	57.2
trnG-GCC-trnR-UCU_p4	AGCCTTCCAAGCTAACGATG	AGGTTTAGAAGACCTTTGTCCT	50.0	40.9	58.0	56.7	210-211 bp	1.0	57.2
trnG-GCC-trnR-UCU_p5	CCTAGCCTTCCAAGCTAACG	AGAAATTGCGTCCAATAGGA	55.0	40.0	58.1	54.7	249-250 bp	1.0	56.9