Hotspot

ndhD

SSC · 120047-121357 · 1311 bp

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Pi 0.0139

MarkerSeek score 38.40

Primer available yes

Label ndhD

Feature Metadata

label_name: ndhD
feature_type: gene
region: SSC
length_bp: 1311
variable_sites: 34
pi: 0.0139
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 38.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhD_p1	TTGGAGTTGGGACCCAAAAG	GGCAAGGTATGATCGTGGTT	50.0	50.0	57.9	58.0	2483-2503 bp	1.0	82.5
ndhD_p2	TTGGAGTTGGGACCCAAAAG	GACCGAAGACTGTGTTGGTT	50.0	50.0	57.9	58.1	2164-2179 bp	1.0	82.5
ndhD_p3	TTTGATTCGCGGGGTTCTAG	GGCAAGGTATGATCGTGGTT	50.0	50.0	58.0	58.0	2180-2201 bp	0.4	57.5
ndhD_p4	TTTGATTCGCGGGGTTCTAG	GACCGAAGACTGTGTTGGTT	50.0	50.0	58.0	58.1	1862-1877 bp	0.4	57.5
ndhD_p5	TTTGATTCGCGGGGTTCTAG	CCACGAACCATTTTCCTTGC	50.0	50.0	58.0	57.9	1642-1655 bp	0.4	57.4