Hotspot

ndhA

SSC · 124081-126186 · 2106 bp

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Pi 0.0116

MarkerSeek score 58.90

Primer available yes

Label ndhA

Feature Metadata

label_name: ndhA
feature_type: gene
region: SSC
length_bp: 2106
variable_sites: 46
pi: 0.0116
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 58.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhA_p1	TGAGTTAGCATAGGGAACAT	GCCGGGTTTTATCAACTTGC	40.0	50.0	53.4	58.0	2323-2338 bp	1.0	45.3
ndhA_p2	TGAGTTAGCATAGGGAACAT	TGCAAATTCTTCCGCAGCTA	40.0	45.0	53.4	58.2	2306-2321 bp	1.0	44.0
ndhA_p3	TGAGTTAGCATAGGGAACAT	CGCCGGGTTTTATCAACTTG	40.0	50.0	53.4	57.7	2324-2339 bp	1.0	43.4
ndhA_p4	TGAGTTAGCATAGGGAACATATCTG	GCCGGGTTTTATCAACTTGC	40.0	50.0	58.0	58.0	2323-2338 bp	1.0	42.2
ndhA_p5	TGAGTTAGCATAGGGAACAT	GCAAATTCTTCCGCAGCTAG	40.0	50.0	53.4	57.5	2305-2320 bp	1.0	42.2