Hotspot

ccsA-ndhD

SSC · 119830-120046 · 217 bp

Back to Schnabelia

Pi 0.0747
MarkerSeek score 80.90
Primer available yes
Label ccsA-ndhD

Feature Metadata

label_name
ccsA-ndhD
feature_type
igs
region
SSC
length_bp
217
variable_sites
28
pi
0.0747
unique_haplotype_count
5
primer_available
yes
alignment_reliability
1.00
markerseek_score
80.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ccsA-ndhD_p1 TGGATATGCTATTTTGGAGTCA GGTTGAAGTTATTCTATCTAATTCT 36.4 28.0 54.9 52.7 340-353 bp 1.0 49.5
ccsA-ndhD_p2 TGGATATGCTATTTTGGAGTCAA GGTTGAAGTTATTCTATCTAATTCT 34.8 28.0 55.7 52.7 340-353 bp 1.0 49.5
ccsA-ndhD_p3 TTGGATATGCTATTTTGGAGTCA GGTTGAAGTTATTCTATCTAATTCT 34.8 28.0 55.7 52.7 341-354 bp 1.0 49.5
ccsA-ndhD_p4 TGGATATGCTATTTTGGAGTCA GGTTGAAGTTATTCTATCTAATTCTT 36.4 26.9 54.9 53.4 340-353 bp 1.0 49.5
ccsA-ndhD_p5 TGGATATGCTATTTTGGAGTCAA GGTTGAAGTTATTCTATCTAATTCTT 34.8 26.9 55.7 53.4 340-353 bp 1.0 49.5