Hotspot

ndhF-rpl32

SSC · 117537-118054 · 518 bp

Back to Schnabelia

Pi 0.0414

MarkerSeek score 75.70

Primer available yes

Label ndhF-rpl32

Feature Metadata

label_name: ndhF-rpl32
feature_type: igs
region: SSC
length_bp: 518
variable_sites: 39
pi: 0.0414
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 75.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF-rpl32_p1	AGTGGAACGAAGGGCAAAAT	TGATGCAGAAGTACGTTTTT	45.0	35.0	58.0	53.5	598-599 bp	1.0	49.6
ndhF-rpl32_p2	AGTGGAACGAAGGGCAAAAT	TTGATGCAGAAGTACGTTTT	45.0	35.0	58.0	53.5	599-600 bp	1.0	49.5
ndhF-rpl32_p3	AGTGGAACGAAGGGCAAAAT	TTTGATGCAGAAGTACGTTT	45.0	35.0	58.0	53.5	600-601 bp	1.0	49.5
ndhF-rpl32_p4	AGTGGAACGAAGGGCAAAAT	TTTTGATGCAGAAGTACGTT	45.0	35.0	58.0	53.5	601-602 bp	1.0	49.5
ndhF-rpl32_p5	AGTGGAACGAAGGGCAAAAT	TTTTTGATGCAGAAGTACGT	45.0	35.0	58.0	53.5	602-603 bp	1.0	49.5