Hotspot

rps12-clpP

LSC · 69744-69874 · 131 bp

Back to Schnabelia

Pi 0.031

MarkerSeek score 67.50

Primer available yes

Label rps12-clpP

Feature Metadata

label_name: rps12-clpP
feature_type: igs
region: LSC
length_bp: 131
variable_sites: 8
pi: 0.031
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 67.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps12-clpP_p1	TGACATTTCGAATGGGCTGT	TATGTCAGCAACAGAAGCCC	45.0	50.0	57.8	57.9	247-250 bp	1.0	82.0
rps12-clpP_p2	TGACATTTCGAATGGGCTGT	GCCCAAGCTTATGGACTTGT	45.0	50.0	57.8	58.2	231-234 bp	1.0	82.0
rps12-clpP_p3	CTAGTACATGTTCCTCGGCG	TATGTCAGCAACAGAAGCCC	55.0	50.0	57.9	57.9	299-302 bp	1.0	81.9
rps12-clpP_p4	CTAGTACATGTTCCTCGGCG	GCCCAAGCTTATGGACTTGT	55.0	50.0	57.9	58.2	283-286 bp	1.0	81.7
rps12-clpP_p5	TTTCGAATGGGCTGTCTTGT	TATGTCAGCAACAGAAGCCC	45.0	50.0	57.7	57.9	242-245 bp	1.0	81.5