Hotspot

petA-psbJ

LSC · 63115-64066 · 952 bp

Back to Schnabelia

Pi 0.0134

MarkerSeek score 59.60

Primer available yes

Label petA-psbJ

Feature Metadata

label_name: petA-psbJ
feature_type: igs
region: LSC
length_bp: 952
variable_sites: 23
pi: 0.0134
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-psbJ_p1	ATCCATTACGTGTCCAAGGC	CCGGATTAGGTTCATCCTTGT	50.0	47.6	58.0	57.4	1003-1104 bp	1.0	72.9
petA-psbJ_p2	GAGAAGGTTCAATTGGCCGA	CCGGATTAGGTTCATCCTTGT	50.0	47.6	58.2	57.4	917-1018 bp	1.0	71.9
petA-psbJ_p3	TCGAGAAGGTTCAATTGGCC	CCGGATTAGGTTCATCCTTGT	50.0	47.6	58.2	57.4	919-1020 bp	1.0	71.9
petA-psbJ_p4	CATTACGTGTCCAAGGCCTT	CCGGATTAGGTTCATCCTTGT	50.0	47.6	58.2	57.4	1000-1101 bp	1.0	71.8
petA-psbJ_p5	AGAAGGTTCAATTGGCCGAA	CCGGATTAGGTTCATCCTTGT	45.0	47.6	57.7	57.4	916-1017 bp	1.0	71.1