Hotspot

trnT-UGU-trnL-UAA

LSC · 47135-47873 · 739 bp

Back to Schnabelia

Pi 0.0242

MarkerSeek score 66.40

Primer available yes

Label trnT-UGU-trnL-UAA

Feature Metadata

label_name: trnT-UGU-trnL-UAA
feature_type: igs
region: LSC
length_bp: 739
variable_sites: 33
pi: 0.0242
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 66.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnT-UGU-trnL-UAA_p1	GCGATGCTCTAACCTCTGAG	CCGTAGCGTCTACCGATTTC	55.0	55.0	57.9	58.2	777-800 bp	1.0	83.9
trnT-UGU-trnL-UAA_p2	GGAATCGAACCGATGACCAT	CCGTAGCGTCTACCGATTTC	50.0	55.0	57.8	58.2	809-832 bp	1.0	82.7
trnT-UGU-trnL-UAA_p3	TAGGAAAAAGCCGGCTATCG	CCGTAGCGTCTACCGATTTC	50.0	55.0	57.8	58.2	828-851 bp	1.0	82.6
trnT-UGU-trnL-UAA_p4	GCGATGCTCTAACCTCTGAG	CGTCTACCGATTTCGCCATA	55.0	50.0	57.9	57.6	771-794 bp	1.0	82.6
trnT-UGU-trnL-UAA_p5	TAGGAAAAAGCCGGCTATCG	CGTCTACCGATTTCGCCATA	50.0	50.0	57.8	57.6	822-845 bp	1.0	81.4