Hotspot

trnG-GCC-trnfM-CAU

LSC · 37187-37334 · 148 bp

Back to Schnabelia

Pi 0.0351

MarkerSeek score 67.00

Primer available yes

Label trnG-GCC-trnfM-CAU

Feature Metadata

label_name: trnG-GCC-trnfM-CAU
feature_type: igs
region: LSC
length_bp: 148
variable_sites: 12
pi: 0.0351
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 67.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnG-GCC-trnfM-CAU_p1	AGAAGATGCGGGTTCGATTC	CTTGAGGTCACGGGTTCAAA	50.0	50.0	58.1	58.0	214-243 bp	1.0	87.4
trnG-GCC-trnfM-CAU_p2	GAGAAGATGCGGGTTCGATT	CTTGAGGTCACGGGTTCAAA	50.0	50.0	58.1	58.0	215-244 bp	1.0	87.4
trnG-GCC-trnfM-CAU_p3	AGAAGATGCGGGTTCGATTC	GTCACGGGTTCAAATCCTGT	50.0	50.0	58.1	58.1	208-237 bp	1.0	87.0
trnG-GCC-trnfM-CAU_p4	GAGAAGATGCGGGTTCGATT	GTCACGGGTTCAAATCCTGT	50.0	50.0	58.1	58.1	209-238 bp	1.0	86.9
trnG-GCC-trnfM-CAU_p5	ATGCGGATATGGTCGAATGG	CTTGAGGTCACGGGTTCAAA	50.0	50.0	57.9	58.0	255-284 bp	1.0	86.4