Hotspot

petN-psbM

LSC · 29615-30581 · 967 bp

Back to Schnabelia

Pi 0.0209

MarkerSeek score 62.40

Primer available yes

Label petN-psbM

Feature Metadata

label_name: petN-psbM
feature_type: igs
region: LSC
length_bp: 967
variable_sites: 39
pi: 0.0209
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 62.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petN-psbM_p1	TCGTAGTGTGGGGAAGAAGT	TCCAAACGAGTCAAAGGGTG	50.0	50.0	58.0	58.0	1414-1585 bp	1.0	86.4
petN-psbM_p2	ATCCTCGTTCGCATCTAGGA	TCCAAACGAGTCAAAGGGTG	50.0	50.0	58.0	58.0	2049-2226 bp	1.0	86.2
petN-psbM_p3	TCCTCGTTCGCATCTAGGAT	TCCAAACGAGTCAAAGGGTG	50.0	50.0	58.0	58.0	2048-2225 bp	1.0	86.2
petN-psbM_p4	TCGTAGTGTGGGGAAGAAGT	GGTGTCGAGTGGCATACTTT	50.0	50.0	58.0	57.9	1398-1569 bp	1.0	86.0
petN-psbM_p5	TCGTAGTGTGGGGAAGAAGT	TCCCTTAATTTCCCCGGGAT	50.0	50.0	58.0	58.1	1534-1704 bp	1.0	85.9