Hotspot

rpoC2

LSC · 26740-31281 · 4542 bp

Back to Schizostachyum

Pi 0.00192

MarkerSeek score 55.10

Primer available yes

Label rpoC2

Feature Metadata

label_name: rpoC2
feature_type: gene
region: LSC
length_bp: 4542
variable_sites: 13
pi: 0.00192
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 55.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoC2_p1	TTCGCTCCACAAGGATCAAG	CAAGAGATCCTGCGGGAAAA	50.0	50.0	57.9	57.9	1291-1387 bp	1.0	80.9
rpoC2_p2	AATCATGTCCAACGGCCATT	CAAGAGATCCTGCGGGAAAA	45.0	50.0	58.1	57.9	1129-1225 bp	1.0	80.8
rpoC2_p3	TTCGCTCCACAAGGATCAAG	ACCAATCCACCTAGTCGACT	50.0	50.0	57.9	57.8	1011-1107 bp	1.0	80.6
rpoC2_p4	ATCATGCGCCCGAATATCAA	CAAGAGATCCTGCGGGAAAA	45.0	50.0	57.8	57.9	1394-1490 bp	1.0	80.1
rpoC2_p5	TCATGCGCCCGAATATCAAT	CAAGAGATCCTGCGGGAAAA	45.0	50.0	57.8	57.9	1393-1489 bp	1.0	80.1