Hotspot

ndhF-rpl32

SSC · 107050-107921 · 872 bp

Back to Schizostachyum

Pi 0.00535
MarkerSeek score 64.80
Primer available yes
Label ndhF-rpl32

Feature Metadata

label_name
ndhF-rpl32
feature_type
igs
region
SSC
length_bp
872
variable_sites
7
pi
0.00535
unique_haplotype_count
3
primer_available
yes
alignment_reliability
1.00
markerseek_score
64.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhF-rpl32_p1 GGAAGTGGGAGAAGAGGGAT TCCAAAGATTTTTACGAATACGCT 55.0 33.3 58.1 57.3 983-1043 bp 1.0 43.4
ndhF-rpl32_p2 GGAAGTGGGAGAAGAGGGAT TGACATCGAAGTACGTTTTT 55.0 35.0 58.1 53.2 955-1015 bp 1.0 43.1
ndhF-rpl32_p3 GGAAGTGGGAGAAGAGGGAT TTGACATCGAAGTACGTTTT 55.0 35.0 58.1 53.2 956-1016 bp 1.0 43.1
ndhF-rpl32_p4 GGAAGTGGGAGAAGAGGGAT TTTGACATCGAAGTACGTTT 55.0 35.0 58.1 53.2 957-1017 bp 1.0 43.1
ndhF-rpl32_p5 GGAAGTGGGAGAAGAGGGAT TTTTGACATCGAAGTACGTT 55.0 35.0 58.1 53.2 958-1018 bp 1.0 43.1