Hotspot

rps15-ndhF

IRb · 104388-104829 · 442 bp

Back to Schizostachyum

Pi 0.00679

MarkerSeek score 60.90

Primer available yes

Label rps15-ndhF

Feature Metadata

label_name: rps15-ndhF
feature_type: igs
region: IRb
length_bp: 442
variable_sites: 4
pi: 0.00679
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps15-ndhF_p1	TCGGAAAGAGGTCTACGAAGA	GGGGAGGGCGAATATCATCT	47.6	55.0	57.9	58.7	634-641 bp	1.0	66.9
rps15-ndhF_p2	ACTTTTGGGAAAACGTCAACG	GGGGAGGGCGAATATCATCT	42.9	55.0	57.9	58.7	614-621 bp	1.0	66.3
rps15-ndhF_p3	TCGGAAAGAGGTCTACGAAGA	AAATATGTAGGGGGAGGGCG	47.6	55.0	57.9	58.9	644-651 bp	1.0	65.1
rps15-ndhF_p4	ACTTTTGGGAAAACGTCAACG	AAATATGTAGGGGGAGGGCG	42.9	55.0	57.9	58.9	624-631 bp	1.0	64.5
rps15-ndhF_p5	TCGGAAAGAGGTCTACGAAGA	GTAGGGGGAGGGCGAATAT	47.6	57.9	57.9	57.9	638-645 bp	1.0	63.9