Hotspot

rpl20-rps12

LSC · 68951-69652 · 702 bp

Back to Schizostachyum

Pi 0.000958
MarkerSeek score 60.10
Primer available yes
Label rpl20-rps12

Feature Metadata

label_name
rpl20-rps12
feature_type
igs
region
LSC
length_bp
702
variable_sites
1
pi
0.000958
unique_haplotype_count
3
primer_available
yes
alignment_reliability
0.99
markerseek_score
60.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl20-rps12_p1 ACGCATTTTTGTTCGTCGTC GAGGAACATGTGCTAGGGTG 45.0 55.0 58.0 58.0 773-779 bp 1.0 80.4
rpl20-rps12_p2 ACGCATTTTTGTTCGTCGTC CGACATGCCAACGGTTAAAC 45.0 50.0 58.0 58.0 868-874 bp 1.0 80.2
rpl20-rps12_p3 ACGCATTTTTGTTCGTCGTC GTCGAGGAACATGTGCTAGG 45.0 55.0 58.0 58.1 776-782 bp 1.0 80.0
rpl20-rps12_p4 GTTTGAGGCAAATGAACGCA GAGGAACATGTGCTAGGGTG 45.0 55.0 57.9 58.0 788-794 bp 1.0 79.4
rpl20-rps12_p5 GTTTGAGGCAAATGAACGCA CGACATGCCAACGGTTAAAC 45.0 50.0 57.9 58.0 883-889 bp 1.0 79.2