Hotspot

psbF-psbE

LSC · 64191-64200 · 10 bp

Back to Schizostachyum

Pi 0.0667

MarkerSeek score 75.00

Primer available yes

Label psbF-psbE

Feature Metadata

label_name: psbF-psbE
feature_type: igs
region: LSC
length_bp: 10
variable_sites: 1
pi: 0.0667
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 75.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbF-psbE_p1	ACAGTAGGTACGGCTAGTCC	GCCGACAAGGAATTCCGTTA	55.0	50.0	58.0	58.3	161 bp	1.0	77.9
psbF-psbE_p2	CCAGCCATCGGACTGTAAAA	GCCGACAAGGAATTCCGTTA	50.0	50.0	57.9	58.3	133 bp	1.0	77.6
psbF-psbE_p3	TGGATGAACTGCATTGCTGA	GCCGACAAGGAATTCCGTTA	45.0	50.0	57.8	58.3	200 bp	1.0	76.6
psbF-psbE_p4	ACAGTAGGTACGGCTAGTCC	AGTCCTCGGCCAAATGAGTA	55.0	50.0	58.0	58.4	192 bp	1.0	76.6
psbF-psbE_p5	CCAGCCATCGGACTGTAAAA	AGTCCTCGGCCAAATGAGTA	50.0	50.0	57.9	58.4	164 bp	1.0	76.2