Hotspot

trnG-UCC-trnT-GGU

LSC · 13886-15860 · 1975 bp

Back to Schizostachyum

Pi 0.00988

MarkerSeek score 68.20

Primer available yes

Label trnG-UCC-trnT-GGU

Feature Metadata

label_name: trnG-UCC-trnT-GGU
feature_type: igs
region: LSC
length_bp: 1975
variable_sites: 28
pi: 0.00988
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 68.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnG-UCC-trnT-GGU_p1	ACGAATCACACTTTTACCACT	CCATGGCATTACTCTACCACC	38.1	52.4	55.5	58.1	2067-2111 bp	1.0	47.6
trnG-UCC-trnT-GGU_p2	ACGAATCACACTTTTACCACT	GACTTACGTCTTACCATGGCA	38.1	47.6	55.5	57.8	2080-2124 bp	1.0	46.8
trnG-UCC-trnT-GGU_p3	ACGAATCACACTTTTACCACT	TGACTTACGTCTTACCATGGC	38.1	47.6	55.5	57.8	2081-2125 bp	1.0	46.8
trnG-UCC-trnT-GGU_p4	AGAACGAATCACACTTTTACCAC	CCATGGCATTACTCTACCACC	39.1	52.4	57.3	58.1	2070-2114 bp	1.0	46.5
trnG-UCC-trnT-GGU_p5	GAACGAATCACACTTTTACCACT	CCATGGCATTACTCTACCACC	39.1	52.4	57.3	58.1	2069-2113 bp	1.0	46.5