Hotspot

NQW19_pgp045

Genome · 56060-147004 · 90945 bp

Back to Schizaea

Pi 0.0955
MarkerSeek score 73.40
Primer available yes
Label NQW19_pgp045

Feature Metadata

label_name
NQW19_pgp045
feature_type
gene
region
Genome
length_bp
90945
variable_sites
21709
pi
0.0955
unique_haplotype_count
8
primer_available
yes
alignment_reliability
0.92
markerseek_score
73.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
NQW19_pgp045_p1 ATCGACGCGTGATAAACCAA GGAAGACAAACTTTCGCACG 45.0 50.0 58.0 58.0 739 bp 0.125 44.8
NQW19_pgp045_p2 CAATCCTCCTGCAAATCGGA GGAAGACAAACTTTCGCACG 50.0 50.0 58.0 58.0 1083 bp 0.125 44.5
NQW19_pgp045_p3 TCCAATCCTCCTGCAAATCG GGAAGACAAACTTTCGCACG 50.0 50.0 58.0 58.0 1085 bp 0.125 44.5
NQW19_pgp045_p4 AGGCTTACTGCATGGCTTTT GGAAGACAAACTTTCGCACG 45.0 50.0 58.1 58.0 913 bp 0.125 44.3
NQW19_pgp045_p5 ATCGACGCGTGATAAACCAA GAGAACCTGGATAACCCCCT 45.0 55.0 58.0 58.1 1065 bp 0.125 44.1