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NQW19_pgp039

Genome · 61563-63023 · 1461 bp

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Pi 0.103

MarkerSeek score 84.80

Primer available yes

Label NQW19_pgp039

Feature Metadata

label_name: NQW19_pgp039
feature_type: gene
region: Genome
length_bp: 1461
variable_sites: 417
pi: 0.103
unique_haplotype_count: 8
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 84.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
NQW19_pgp039_p1	CTTCATGCTCGTCGAAAGGA	TTCGTTGAATTGGGGCTTCA	50.0	45.0	58.0	58.0	2751-2763 bp	0.375	63.8
NQW19_pgp039_p2	TCTTCATGCTCGTCGAAAGG	TTCGTTGAATTGGGGCTTCA	50.0	45.0	58.0	58.0	2752-2764 bp	0.375	63.8
NQW19_pgp039_p3	TTTGATCTGGGAGGCAGAGA	TTCGTTGAATTGGGGCTTCA	50.0	45.0	58.0	58.0	2827-2833 bp	0.375	59.0
NQW19_pgp039_p4	CTAAGTCCCGTTGAAGGTCG	TTCGTTGAATTGGGGCTTCA	55.0	45.0	58.0	58.0	2740-2742 bp	0.25	52.7
NQW19_pgp039_p5	CTTCATGCTCGTCGAAAGGA	GAGTGAGGAGAGGGAGAGAC	50.0	60.0	58.0	57.9	2708 bp	0.125	44.5