Hotspot

NQW19_pgp038

Genome · 63233-64401 · 1169 bp

Back to Schizaea

Pi 0.109

MarkerSeek score 84.40

Primer available yes

Label NQW19_pgp038

Feature Metadata

label_name: NQW19_pgp038
feature_type: gene
region: Genome
length_bp: 1169
variable_sites: 345
pi: 0.109
unique_haplotype_count: 8
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 84.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
NQW19_pgp038_p1	TCGCAAACAAGGTATCTCGG	ACGGTACAGACGACGATTTG	50.0	50.0	58.0	58.0	1879-1899 bp	0.625	71.6
NQW19_pgp038_p2	TCGCAAACAAGGTATCTCGG	CGATCGAGTATCAGCAGCTC	50.0	55.0	58.0	58.0	1698-1718 bp	0.625	71.4
NQW19_pgp038_p3	TCGCAAACAAGGTATCTCGG	CTCGATCGAGTATCAGCAGC	50.0	55.0	58.0	58.0	1700-1720 bp	0.625	71.4
NQW19_pgp038_p4	TCGCAAACAAGGTATCTCGG	AACGGATCTATCTGGGGGAG	50.0	55.0	58.0	58.0	1486-1506 bp	0.375	64.7
NQW19_pgp038_p5	TCGCAAACAAGGTATCTCGG	GAACGGATCTATCTGGGGGA	50.0	55.0	58.0	58.0	1507 bp	0.125	44.9