Hotspot

NQW19_pgp027

Genome · 71279-72852 · 1574 bp

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Pi 0.121
MarkerSeek score 84.60
Primer available yes
Label NQW19_pgp027

Feature Metadata

label_name
NQW19_pgp027
feature_type
gene
region
Genome
length_bp
1574
variable_sites
507
pi
0.121
unique_haplotype_count
8
primer_available
yes
alignment_reliability
1.00
markerseek_score
84.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
NQW19_pgp027_p1 GGAGTAACACGCCCTGTAAG AAACTGGGAACGGGAGAAAC 55.0 50.0 58.0 58.0 2406 bp 0.5 65.7
NQW19_pgp027_p2 GGAGTAACACGCCCTGTAAG TTTCGTGTATGTCGCAGGAG 55.0 50.0 58.0 58.0 2183 bp 0.25 52.7
NQW19_pgp027_p3 CCCGTCCATTGTGAATAGCA TTTCGTGTATGTCGCAGGAG 50.0 50.0 58.0 58.0 1891 bp 0.25 52.7
NQW19_pgp027_p4 GGAGTAACACGCCCTGTAAG AAGAGGGAACTACACGGGAA 55.0 50.0 58.0 58.0 2485 bp 0.125 44.8
NQW19_pgp027_p5 GGAGTAACACGCCCTGTAAG AGAGGGAACTACACGGGAAA 55.0 50.0 58.0 58.0 2484 bp 0.125 44.8