Hotspot

NQW19_pgp005-NQW19_pgr004

Genome · 146120-149758 · 3639 bp

Back to Schizaea

Pi 0.126

MarkerSeek score 67.10

Primer available yes

Label NQW19_pgp005-NQW19_pgr004

Feature Metadata

label_name: NQW19_pgp005-NQW19_pgr004
feature_type: igs
region: Genome
length_bp: 3639
variable_sites: 823
pi: 0.126
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 0.76
markerseek_score: 67.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
NQW19_pgp005-NQW19_pgr004_p1	CGTTGGTTTATAGGGCGACA	TCTATCACCACTGGTTTGCG	50.0	50.0	58.0	57.9	675 bp	0.125	44.2
NQW19_pgp005-NQW19_pgr004_p2	ACGTTGGTTTATAGGGCGAC	TCTATCACCACTGGTTTGCG	50.0	50.0	58.0	57.9	676 bp	0.125	44.1
NQW19_pgp005-NQW19_pgr004_p3	GAACAAACACGCCAACTAGC	TCTATCACCACTGGTTTGCG	50.0	50.0	58.0	57.9	636 bp	0.125	44.0
NQW19_pgp005-NQW19_pgr004_p4	GTACCTGGTCCCTTTCGAAC	TCTATCACCACTGGTTTGCG	55.0	50.0	57.9	57.9	735 bp	0.125	43.5
NQW19_pgp005-NQW19_pgr004_p5	CGTTGGTTTATAGGGCGACA	CTATCACCACTGGTTTGCGT	50.0	50.0	58.0	58.2	674 bp	0.125	43.4