Hotspot

NQW19_pgp020-NQW19_pgt022

Genome · 100976-101714 · 739 bp

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Pi 0.108
MarkerSeek score 84.30
Primer available yes
Label NQW19_pgp020-NQW19_pgt022

Feature Metadata

label_name
NQW19_pgp020-NQW19_pgt022
feature_type
igs
region
Genome
length_bp
739
variable_sites
223
pi
0.108
unique_haplotype_count
8
primer_available
yes
alignment_reliability
0.98
markerseek_score
84.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
NQW19_pgp020-NQW19_pgt022_p1 AAAGGGATAAGGACGGGACA TCCACATCGATTCGTGAACC 50.0 50.0 58.0 58.0 1682-1734 bp 0.75 81.7
NQW19_pgp020-NQW19_pgt022_p2 AAGGGATAAGGACGGGACAA TCCACATCGATTCGTGAACC 50.0 50.0 58.0 58.0 1681-1733 bp 0.75 81.7
NQW19_pgp020-NQW19_pgt022_p3 AGGGATAAGGACGGGACAAA TCCACATCGATTCGTGAACC 50.0 50.0 58.0 58.0 1680-1732 bp 0.75 81.7
NQW19_pgp020-NQW19_pgt022_p4 AAGGGATAAGGACGGGACAA TTGAGTCACCAACCCCATTG 50.0 50.0 58.0 58.0 1967-1987 bp 0.625 69.4
NQW19_pgp020-NQW19_pgt022_p5 AGGGATAAGGACGGGACAAA TTGAGTCACCAACCCCATTG 50.0 50.0 58.0 58.0 1966-1986 bp 0.625 69.4