Hotspot

NQW19_pgp046-NQW19_pgp045

Genome · 55265-56059 · 795 bp

Back to Schizaea

Pi 0.129

MarkerSeek score 83.80

Primer available yes

Label NQW19_pgp046-NQW19_pgp045

Feature Metadata

label_name: NQW19_pgp046-NQW19_pgp045
feature_type: igs
region: Genome
length_bp: 795
variable_sites: 272
pi: 0.129
unique_haplotype_count: 8
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 83.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
NQW19_pgp046-NQW19_pgp045_p1	AACAATCTCGAATGAGCCCC	GTGTCATGGAAGCAGAGGAG	50.0	55.0	58.0	58.0	1438-1477 bp	0.75	80.4
NQW19_pgp046-NQW19_pgp045_p2	TTTACTCTCTGCTGGTTCGC	GTGTCATGGAAGCAGAGGAG	50.0	55.0	57.9	58.0	1465-1504 bp	0.75	80.1
NQW19_pgp046-NQW19_pgp045_p3	CTCGGTATCCAAAATGGCCA	GTGTCATGGAAGCAGAGGAG	50.0	55.0	57.9	58.0	1665-1694 bp	0.625	72.5
NQW19_pgp046-NQW19_pgp045_p4	AACAATCTCGAATGAGCCCC	AATCCATGTTCGAAGGACGG	50.0	50.0	58.0	58.0	1428 bp	0.125	44.5
NQW19_pgp046-NQW19_pgp045_p5	CTCGGTATCCAAAATGGCCA	AATCCATGTTCGAAGGACGG	50.0	50.0	57.9	58.0	1645 bp	0.125	44.4