Hotspot

NQW19_pgp062-NQW19_pgp061

Genome · 42388-43023 · 636 bp

Back to Schizaea

Pi 0.116

MarkerSeek score 83.50

Primer available yes

Label NQW19_pgp062-NQW19_pgp061

Feature Metadata

label_name: NQW19_pgp062-NQW19_pgp061
feature_type: igs
region: Genome
length_bp: 636
variable_sites: 210
pi: 0.116
unique_haplotype_count: 8
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 83.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
NQW19_pgp062-NQW19_pgp061_p1	AGAACCGTTTTTCCCACTCC	CGCGGAGACACTCATAAACA	50.0	50.0	58.0	58.0	1746-1755 bp	0.625	72.5
NQW19_pgp062-NQW19_pgp061_p2	CGTAGACTAGCGCCACTTTT	CGCGGAGACACTCATAAACA	50.0	50.0	58.0	58.0	1913-1922 bp	0.625	72.3
NQW19_pgp062-NQW19_pgp061_p3	TTGAGCCAACTCTCATACGC	CGCGGAGACACTCATAAACA	50.0	50.0	58.0	58.0	1958-1967 bp	0.625	72.1
NQW19_pgp062-NQW19_pgp061_p4	CCCATGAGCCTTGGCAATAT	CGCGGAGACACTCATAAACA	50.0	50.0	58.0	58.0	1786-1795 bp	0.5	67.1
NQW19_pgp062-NQW19_pgp061_p5	CCCATGAGCCTTGGCAATAT	ATCTCGTTCAACCTGGATGC	50.0	50.0	58.0	58.0	1683-1692 bp	0.375	57.9