Hotspot

rps16

LSC · 4488-5588 · 1101 bp

Back to Schizachyrium

Pi 0.00994

MarkerSeek score 50.40

Primer available yes

Label rps16

Feature Metadata

label_name: rps16
feature_type: gene
region: LSC
length_bp: 1101
variable_sites: 59
pi: 0.00994
unique_haplotype_count: 28
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 50.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps16_p1	GCAAGAGGAGCAACCCTATC	GAGGTTCGAATCCTTCCGTC	55.0	55.0	58.0	58.1	2099-2948 bp	1.0	85.2
rps16_p2	GCAAGAGGAGCAACCCTATC	ACCCCGTATCTATGGAGTGG	55.0	55.0	58.0	58.0	1797-2646 bp	0.625	69.1
rps16_p3	TGAGGATCAGTCGTGGTCTT	ACCCCGTATCTATGGAGTGG	50.0	55.0	58.1	58.0	2192-2982 bp	0.3	55.1
rps16_p4	GCAAGAGGAGCAACCCTATC	GCCCTGCACAACACAAAAAT	55.0	45.0	58.0	58.0	2043-2048 bp	0.1	44.2
rps16_p5	TGAGGATCAGTCGTGGTCTT	GCCCTGCACAACACAAAAAT	50.0	45.0	58.1	58.0	2439-2443 bp	0.1	43.9