Hotspot

rpl33-rps18

LSC · 65098-65375 · 278 bp

Back to Schizachyrium

Pi 0.0212

MarkerSeek score 60.30

Primer available yes

Label rpl33-rps18

Feature Metadata

label_name: rpl33-rps18
feature_type: igs
region: LSC
length_bp: 278
variable_sites: 30
pi: 0.0212
unique_haplotype_count: 31
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 60.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl33-rps18_p1	AAAGGAGGTTCATGGCCAAG	TCGAGAACGCCTACGAAAAG	50.0	50.0	58.1	58.0	609-656 bp	0.975	88.0
rpl33-rps18_p2	GAAAGGAGGTTCATGGCCAA	TCGAGAACGCCTACGAAAAG	50.0	50.0	58.1	58.0	610-657 bp	0.975	88.0
rpl33-rps18_p3	GAAAGGAGGTTCATGGCCAA	ACGCCTACGAAAAGGTTGTT	50.0	45.0	58.1	58.0	604-651 bp	0.975	88.0
rpl33-rps18_p4	AAAGGAGGTTCATGGCCAAG	ACGCCTACGAAAAGGTTGTT	50.0	45.0	58.1	58.0	603-650 bp	0.975	87.9
rpl33-rps18_p5	AAAGGAGGTTCATGGCCAAG	AACGCCTACGAAAAGGTTGT	50.0	45.0	58.1	58.0	604-649 bp	0.925	85.8