Hotspot

petA-psbJ

LSC · 60576-61474 · 899 bp

Back to Schizachyrium

Pi 0.0131

MarkerSeek score 70.90

Primer available yes

Label petA-psbJ

Feature Metadata

label_name: petA-psbJ
feature_type: igs
region: LSC
length_bp: 899
variable_sites: 62
pi: 0.0131
unique_haplotype_count: 29
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 70.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-psbJ_p1	GGCCTTTTGTTCTTCTTCGC	ACGGTTCGTATTCTGGATTGG	50.0	47.6	57.9	58.1	971-1059 bp	1.0	79.7
petA-psbJ_p2	GGCCTTTTGTTCTTCTTCGC	GGTTCGTATTCTGGATTGGGT	50.0	47.6	57.9	57.7	969-1057 bp	1.0	78.2
petA-psbJ_p3	GGCCTTTTGTTCTTCTTCGC	CGGTTCGTATTCTGGATTGG	50.0	50.0	57.9	56.3	970-1058 bp	1.0	75.1
petA-psbJ_p4	CGTCCAAGGCCTTTTGTTCT	ACGGTTCGTATTCTGGATTGG	50.0	47.6	58.7	58.1	978-1066 bp	1.0	74.6
petA-psbJ_p5	GGCCTTTTGTTCTTCTTCGC	CGGTTCGTATTCTGGATTGGG	50.0	52.4	57.9	58.8	970-1058 bp	1.0	74.2