Hotspot

rbcL-psaI

LSC · 56451-57270 · 820 bp

Back to Schizachyrium

Pi 0.00923

MarkerSeek score 70.70

Primer available yes

Label rbcL-psaI

Feature Metadata

label_name: rbcL-psaI
feature_type: igs
region: LSC
length_bp: 820
variable_sites: 61
pi: 0.00923
unique_haplotype_count: 33
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 70.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rbcL-psaI_p1	TGCAAATGGAGTGCTGAACT	GTCATTGCAATTGCCGGAAA	45.0	45.0	58.0	57.9	945-1124 bp	1.0	88.4
rbcL-psaI_p2	GCCGCAGCTTGTGAAATATG	GTCATTGCAATTGCCGGAAA	50.0	45.0	58.2	57.9	924-1103 bp	1.0	87.2
rbcL-psaI_p3	TGCAAATGGAGTGCTGAACT	GAAGTCATTGCAATTGCCGG	45.0	50.0	58.0	58.4	948-1127 bp	1.0	86.0
rbcL-psaI_p4	TGCAAATGGAGTGCTGAACT	GGAAGTCATTGCAATTGCCG	45.0	50.0	58.0	58.4	949-1128 bp	1.0	86.0
rbcL-psaI_p5	GCCGCAGCTTGTGAAATATG	GAAGTCATTGCAATTGCCGG	50.0	50.0	58.2	58.4	927-1106 bp	1.0	84.8