Hotspot

trnC-GCA-rpoB

LSC · 18808-20049 · 1242 bp

Back to Schizachyrium

Pi 0.0121

MarkerSeek score 62.60

Primer available yes

Label trnC-GCA-rpoB

Feature Metadata

label_name: trnC-GCA-rpoB
feature_type: igs
region: LSC
length_bp: 1242
variable_sites: 91
pi: 0.0121
unique_haplotype_count: 38
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 62.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnC-GCA-rpoB_p1	CTGCCCCGCTTTCTATCTTT	CCCCACCTACACAAGCAAAT	50.0	50.0	58.0	58.1	1892-2101 bp	1.0	87.6
trnC-GCA-rpoB_p2	AAAGGATTTGCAGTCCCCTG	CCCCACCTACACAAGCAAAT	50.0	50.0	58.1	58.1	1792-2001 bp	1.0	87.4
trnC-GCA-rpoB_p3	GACCTAAACCCTACTGCTGC	CCCCACCTACACAAGCAAAT	55.0	50.0	58.0	58.1	1908-2117 bp	0.975	86.7
trnC-GCA-rpoB_p4	TGCCCCGCTTTCTATCTTTC	CCCCACCTACACAAGCAAAT	50.0	50.0	58.0	58.1	1891-2100 bp	0.975	86.6
trnC-GCA-rpoB_p5	GACCTAAACCCTACTGCTGC	GCTTTTGCACATCGAAACCA	55.0	45.0	58.0	57.9	1491-1700 bp	0.95	85.7