Hotspot

trnG-GCC-trnfM-CAU

LSC · 12027-12405 · 379 bp

Back to Schizachyrium

Pi 0.0229

MarkerSeek score 64.30

Primer available yes

Label trnG-GCC-trnfM-CAU

Feature Metadata

label_name: trnG-GCC-trnfM-CAU
feature_type: igs
region: LSC
length_bp: 379
variable_sites: 46
pi: 0.0229
unique_haplotype_count: 32
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 64.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnG-GCC-trnfM-CAU_p1	AGGAGAAGATACGGGTTCGA	CGAGGCTCATAACCTTGAGG	50.0	55.0	57.6	57.8	303-478 bp	1.0	86.7
trnG-GCC-trnfM-CAU_p2	GTAGCGAGCGTAGTTCAGTG	CGAGGCTCATAACCTTGAGG	55.0	55.0	58.5	57.8	342-517 bp	1.0	86.4
trnG-GCC-trnfM-CAU_p3	ATGTAGCGAGCGTAGTTCAG	CGAGGCTCATAACCTTGAGG	50.0	55.0	57.5	57.8	344-519 bp	1.0	86.3
trnG-GCC-trnfM-CAU_p4	AGGAGAAGATACGGGTTCGA	CAGTTTGGTAGCTCACGAGG	50.0	55.0	57.6	58.3	318-493 bp	1.0	86.3
trnG-GCC-trnfM-CAU_p5	GTAGCGAGCGTAGTTCAGTG	CAGTTTGGTAGCTCACGAGG	55.0	55.0	58.5	58.3	357-532 bp	1.0	86.0