Hotspot

trnS-GCU-psbD

LSC · 7505-8462 · 958 bp

Back to Schizachyrium

Pi 0.00773

MarkerSeek score 65.40

Primer available yes

Label trnS-GCU-psbD

Feature Metadata

label_name: trnS-GCU-psbD
feature_type: igs
region: LSC
length_bp: 958
variable_sites: 62
pi: 0.00773
unique_haplotype_count: 33
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 65.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-GCU-psbD_p1	ATTAGCAATCCGCCGCTTTA	ATAAGCCGGACCATCCTACA	45.0	50.0	58.0	57.9	1089-1140 bp	1.0	87.2
trnS-GCU-psbD_p2	ATTAGCAATCCGCCGCTTTA	TAAGCCGGACCATCCTACAA	45.0	50.0	58.0	58.1	1088-1139 bp	1.0	86.9
trnS-GCU-psbD_p3	ATTAGCAATCCGCCGCTTTA	GCCGGACCATCCTACAAAAA	45.0	50.0	58.0	58.2	1085-1136 bp	1.0	86.6
trnS-GCU-psbD_p4	ATTAGCAATCCGCCGCTTTA	ACAAAAACGAAACGGTCCCT	45.0	45.0	58.0	58.2	1072-1123 bp	1.0	86.1
trnS-GCU-psbD_p5	GCTTTAGTCCACTCAGCCAT	ATAAGCCGGACCATCCTACA	50.0	50.0	57.6	57.9	1075-1126 bp	1.0	84.3