Hotspot

matK-trnK-UUU

LSC · 3206-3896 · 691 bp

Back to Schizachyrium

Pi 0.00837
MarkerSeek score 63.60
Primer available yes
Label matK-trnK-UUU

Feature Metadata

label_name
matK-trnK-UUU
feature_type
igs
region
LSC
length_bp
691
variable_sites
41
pi
0.00837
unique_haplotype_count
35
primer_available
yes
alignment_reliability
1.00
markerseek_score
63.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
matK-trnK-UUU_p1 ACGAAGTAGTGTTGACGAGA GAGCGTCCGGTGTAATTTCA 45.0 50.0 56.3 58.3 839-860 bp 0.975 68.2
matK-trnK-UUU_p2 ACGAAGTAGTGTTGACGAGA ATGTTTTGACAGGAGCGTCC 45.0 50.0 56.3 58.5 851-872 bp 1.0 68.0
matK-trnK-UUU_p3 ACGAAGTAGTGTTGACGAGA AGCGTCCGGTGTAATTTCAT 45.0 45.0 56.3 57.6 838-859 bp 0.975 67.3
matK-trnK-UUU_p4 TGAAAGAGAAGTGGGTAGACG GAGCGTCCGGTGTAATTTCA 47.6 50.0 57.1 58.3 857-878 bp 0.975 66.4
matK-trnK-UUU_p5 AGAGAAGTGGGTAGACGAAG GAGCGTCCGGTGTAATTTCA 50.0 50.0 56.0 58.3 853-874 bp 0.975 66.0