Hotspot

rpoB

LSC · 20313-23543 · 3231 bp

Back to Schizachne

Pi 0.00217

MarkerSeek score 41.50

Primer available yes

Label rpoB

Feature Metadata

label_name: rpoB
feature_type: gene
region: LSC
length_bp: 3231
variable_sites: 7
pi: 0.00217
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 41.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoB_p1	TGCGAATCGGGCTTTAATGA	TTCCTAAAGCAAGTTCCCCC	45.0	50.0	58.0	57.4	344 bp	1.0	75.7
rpoB_p2	GAACGCCAAACAGCTCTAGA	TTCCTAAAGCAAGTTCCCCC	50.0	50.0	57.9	57.4	256 bp	1.0	75.5
rpoB_p3	TCCGCTTTCTAAGTCCGAGA	TTCCTAAAGCAAGTTCCCCC	50.0	50.0	58.2	57.4	299 bp	1.0	74.7
rpoB_p4	TGCGAATCGGGCTTTAATGA	ATTGTAACCTTCCCATGGCA	45.0	45.0	58.0	57.1	385 bp	1.0	73.0
rpoB_p5	TGCGAATCGGGCTTTAATGA	TTGTAACCTTCCCATGGCAT	45.0	45.0	58.0	57.1	384 bp	1.0	73.0