Hotspot

rps15-ndhF

IRb · 101496-101955 · 460 bp

Back to Schizachne

Pi 0.0109

MarkerSeek score 62.50

Primer available yes

Label rps15-ndhF

Feature Metadata

label_name: rps15-ndhF
feature_type: igs
region: IRb
length_bp: 460
variable_sites: 5
pi: 0.0109
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 62.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps15-ndhF_p1	CTTTTGGGAAAACGTCGACG	AAATATGTAGGGGGAGGGCG	50.0	55.0	58.0	58.9	644-646 bp	1.0	73.3
rps15-ndhF_p2	CTTTTGGGAAAACGTCGACG	GGAGGGCGAATCTCGTCTTA	50.0	55.0	58.0	59.0	632-634 bp	1.0	73.0
rps15-ndhF_p3	CTTTTGGGAAAACGTCGACG	GAGGGCGAATCTCGTCTTAT	50.0	50.0	58.0	56.9	631-633 bp	1.0	72.1
rps15-ndhF_p4	TCGACGTTTGCTGGCTTATT	AAATATGTAGGGGGAGGGCG	45.0	55.0	58.2	58.9	630-632 bp	1.0	71.8
rps15-ndhF_p5	TCGACGTTTGCTGGCTTATT	GGAGGGCGAATCTCGTCTTA	45.0	55.0	58.2	59.0	618-620 bp	1.0	71.6