Hotspot

rpl22-rps19

IRb · 80719-80802 · 84 bp

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Pi 0.0238

MarkerSeek score 66.20

Primer available yes

Label rpl22-rps19

Feature Metadata

label_name: rpl22-rps19
feature_type: igs
region: IRb
length_bp: 84
variable_sites: 2
pi: 0.0238
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 66.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl22-rps19_p1	TCCTTGCGAGTTTCCTTAGC	CAAATTGGGGGAATTCGTGC	50.0	50.0	57.9	58.0	239 bp	1.0	79.8
rpl22-rps19_p2	TCCTTGCGAGTTTCCTTAGC	GCAAATTGGGGGAATTCGTG	50.0	50.0	57.9	58.0	240 bp	1.0	79.8
rpl22-rps19_p3	TCCTTGCGAGTTTCCTTAGC	ATGGTAGGTCGCAAATTGGG	50.0	50.0	57.9	58.2	250 bp	1.0	78.0
rpl22-rps19_p4	TCCTTGCGAGTTTCCTTAGC	GTCGCAAATTGGGGGAATTC	50.0	50.0	57.9	57.7	243 bp	1.0	77.6
rpl22-rps19_p5	CGAGTTTCCTTAGCCCACTT	CAAATTGGGGGAATTCGTGC	50.0	50.0	57.5	58.0	233 bp	1.0	76.8