Hotspot

rps3-rpl22

LSC · 80212-80268 · 57 bp

Back to Schizachne

Pi 0.0351

MarkerSeek score 72.70

Primer available yes

Label rps3-rpl22

Feature Metadata

label_name: rps3-rpl22
feature_type: igs
region: LSC
length_bp: 57
variable_sites: 2
pi: 0.0351
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 72.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps3-rpl22_p1	ACCAAGTCTGAAACCGAGTG	AAAATTCAGACCTCGGGCTC	50.0	50.0	57.8	57.9	193-199 bp	1.0	78.0
rps3-rpl22_p2	CCAAGTCTGAAACCGAGTGG	AAAATTCAGACCTCGGGCTC	55.0	50.0	58.5	57.9	192-198 bp	1.0	76.0
rps3-rpl22_p3	CAAGTCTGAAACCGAGTGGA	AAAATTCAGACCTCGGGCTC	50.0	50.0	57.5	57.9	191-197 bp	1.0	75.7
rps3-rpl22_p4	ACCAAGTCTGAAACCGAGTG	AAAAATTCAGACCTCGGGCT	50.0	45.0	57.8	57.4	194-200 bp	1.0	74.2
rps3-rpl22_p5	CCAAGTCTGAAACCGAGTGG	AAAAATTCAGACCTCGGGCT	55.0	45.0	58.5	57.4	193-199 bp	1.0	72.2