Hotspot

psbH-petB

LSC · 71315-72183 · 869 bp

Back to Schizachne

Pi 0.00345

MarkerSeek score 60.70

Primer available yes

Label psbH-petB

Feature Metadata

label_name: psbH-petB
feature_type: igs
region: LSC
length_bp: 869
variable_sites: 3
pi: 0.00345
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbH-petB_p1	CGCAATGGCTTTATTCGCAA	TGCCTGAATCTCAAGACGTT	45.0	45.0	58.1	57.2	1056-1059 bp	1.0	72.8
psbH-petB_p2	CGCAATGGCTTTATTCGCAA	TTGCCTGAATCTCAAGACGT	45.0	45.0	58.1	57.2	1057-1060 bp	1.0	72.8
psbH-petB_p3	GGTCGCAATGGCTTTATTCG	TGCCTGAATCTCAAGACGTT	50.0	45.0	57.9	57.2	1059-1062 bp	1.0	72.7
psbH-petB_p4	GGTCGCAATGGCTTTATTCG	TTGCCTGAATCTCAAGACGT	50.0	45.0	57.9	57.2	1060-1063 bp	1.0	72.7
psbH-petB_p5	CCTTTTATGGGGGTCGCAAT	TGCCTGAATCTCAAGACGTT	50.0	45.0	58.2	57.2	1070-1073 bp	1.0	71.7