Hotspot

ndhC-trnV

LSC · 50624-51483 · 860 bp

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Pi 0.00581

MarkerSeek score 57.00

Primer available yes

Label ndhC-trnV

Feature Metadata

label_name: ndhC-trnV
feature_type: igs
region: LSC
length_bp: 860
variable_sites: 5
pi: 0.00581
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 57.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhC-trnV_p1	CTAACCGGGGCTAAAAGTCC	GGTTCGAATCCGTATAGCCC	55.0	55.0	58.0	57.9	986 bp	1.0	79.5
ndhC-trnV_p2	CTAACCGGGGCTAAAAGTCC	CGGTTCGAATCCGTATAGCC	55.0	55.0	58.0	58.3	987 bp	1.0	77.7
ndhC-trnV_p3	TAACCGGGGCTAAAAGTCCT	GGTTCGAATCCGTATAGCCC	50.0	55.0	58.3	57.9	985 bp	1.0	77.1
ndhC-trnV_p4	CACTAACCGGGGCTAAAAGT	GGTTCGAATCCGTATAGCCC	50.0	55.0	57.5	57.9	988 bp	1.0	75.9
ndhC-trnV_p5	TAACCGGGGCTAAAAGTCCT	CGGTTCGAATCCGTATAGCC	50.0	55.0	58.3	58.3	986 bp	1.0	75.2