Hotspot

trnC-rpoB

LSC · 19207-20312 · 1106 bp

Back to Schizachne

Pi 0.00452

MarkerSeek score 61.30

Primer available yes

Label trnC-rpoB

Feature Metadata

label_name: trnC-rpoB
feature_type: igs
region: LSC
length_bp: 1106
variable_sites: 5
pi: 0.00452
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnC-rpoB_p1	AAAGGATTTGCAGTCCCCTG	CCGGGTATTGTGGACATTCC	50.0	55.0	58.1	58.3	1190-1202 bp	1.0	77.2
trnC-rpoB_p2	AAAGGATTTGCAGTCCCCTG	CGGGTATTGTGGACATTCCC	50.0	55.0	58.1	58.3	1189-1201 bp	1.0	77.2
trnC-rpoB_p3	AAAGGATTTGCAGTCCCCTG	AAGTTCTTCTGCCAAGCCTT	50.0	45.0	58.1	57.6	1254-1266 bp	1.0	76.8
trnC-rpoB_p4	AAAGGATTTGCAGTCCCCTG	AGTTCTTCTGCCAAGCCTTG	50.0	50.0	58.1	58.4	1253-1265 bp	1.0	76.7
trnC-rpoB_p5	AAAGGATTTGCAGTCCCCTG	CAAGTTCTTCTGCCAAGCCT	50.0	50.0	58.1	58.4	1255-1267 bp	1.0	76.7