Hotspot

trnG-trnT

LSC · 13720-15288 · 1569 bp

Back to Schizachne

Pi 0.00511

MarkerSeek score 60.70

Primer available yes

Label trnG-trnT

Feature Metadata

label_name: trnG-trnT
feature_type: igs
region: LSC
length_bp: 1569
variable_sites: 8
pi: 0.00511
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnG-trnT_p1	ACGAATCACACTTTTACCACT	ACGTCTTACCACGGCATTAC	38.1	50.0	55.5	58.0	1711-1713 bp	1.0	52.2
trnG-trnT_p2	AGAACGAATCACACTTTTACCAC	ACGTCTTACCACGGCATTAC	39.1	50.0	57.3	58.0	1714-1716 bp	1.0	51.1
trnG-trnT_p3	GAACGAATCACACTTTTACCACT	ACGTCTTACCACGGCATTAC	39.1	50.0	57.3	58.0	1713-1715 bp	1.0	51.1
trnG-trnT_p4	AACGAATCACACTTTTACCACT	ACGTCTTACCACGGCATTAC	36.4	50.0	56.2	58.0	1712-1714 bp	1.0	50.3
trnG-trnT_p5	ACGAATCACACTTTTACCACT	CGTCTTACCACGGCATTACT	38.1	50.0	55.5	57.7	1710-1712 bp	1.0	49.9