Hotspot

trnG-trnfM

LSC · 12316-12809 · 494 bp

Back to Schizachne

Pi 0.00811

MarkerSeek score 61.40

Primer available yes

Label trnG-trnfM

Feature Metadata

label_name: trnG-trnfM
feature_type: igs
region: LSC
length_bp: 494
variable_sites: 4
pi: 0.00811
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnG-trnfM_p1	GTCGACCCAAGCGGATATAC	CGAGGCTCATAACCTTGAGG	55.0	55.0	57.9	57.8	651-652 bp	1.0	78.3
trnG-trnfM_p2	GCGAGCGTAGTTCAATGGTA	CGAGGCTCATAACCTTGAGG	50.0	55.0	58.1	57.8	611-612 bp	1.0	78.1
trnG-trnfM_p3	GTCGACCCAAGCGGATATAC	CAGTTTGGTAGCTCACGAGG	55.0	55.0	57.9	58.3	666-667 bp	1.0	77.8
trnG-trnfM_p4	GCGAGCGTAGTTCAATGGTA	CAGTTTGGTAGCTCACGAGG	50.0	55.0	58.1	58.3	626-627 bp	1.0	77.7
trnG-trnfM_p5	GTCGACCCAAGCGGATATAC	TTTTTCGTCCACCAGTTCCC	55.0	50.0	57.9	58.3	708-709 bp	1.0	77.5