Hotspot

ycf2

LSC · 88445-95476 · 7032 bp

Back to Schisandra

Pi 0.0139

MarkerSeek score 50.10

Primer available yes

Label ycf2

Feature Metadata

label_name: ycf2
feature_type: gene
region: LSC
length_bp: 7032
variable_sites: 204
pi: 0.0139
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 50.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf2_p1	GAACCAAGGAATCCGTTCGA	AACCAACAACTCGAAGTGCT	50.0	45.0	57.9	58.0	591-693 bp	1.0	90.1
ycf2_p2	GAACCAAGGAATCCGTTCGA	ACCAACAACTCGAAGTGCTT	50.0	45.0	57.9	58.0	590-692 bp	1.0	90.1
ycf2_p3	TCGGTCGCTCAAGATCTTTG	AACCAACAACTCGAAGTGCT	50.0	45.0	58.0	58.0	780-888 bp	1.0	89.8
ycf2_p4	TCGGTCGCTCAAGATCTTTG	ACCAACAACTCGAAGTGCTT	50.0	45.0	58.0	58.0	779-887 bp	1.0	89.8
ycf2_p5	GGTCGCTCAAGATCTTTGGT	ACCAACAACTCGAAGTGCTT	50.0	45.0	57.9	58.0	777-885 bp	1.0	89.1