Hotspot

rps15-ycf1

SSC · 125625-126007 · 383 bp

Back to Schisandra

Pi 0.0264

MarkerSeek score 63.50

Primer available yes

Label rps15-ycf1

Feature Metadata

label_name: rps15-ycf1
feature_type: igs
region: SSC
length_bp: 383
variable_sites: 22
pi: 0.0264
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 63.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps15-ycf1_p1	CCGCAGACCTCTCTGAGATA	GAACGACATGGAAGGATGCT	55.0	50.0	58.0	58.0	894-946 bp	1.0	86.4
rps15-ycf1_p2	TTTGGCTCGCGAATATCCAA	GAACGACATGGAAGGATGCT	45.0	50.0	58.0	58.0	995-1047 bp	1.0	86.2
rps15-ycf1_p3	AACACCATGCATTGATGGGT	GAACGACATGGAAGGATGCT	45.0	50.0	58.1	58.0	1184-1236 bp	1.0	85.5
rps15-ycf1_p4	TACGACTCAGCTCCAGCATA	GAACGACATGGAAGGATGCT	50.0	50.0	57.9	58.0	1440-1492 bp	1.0	84.6
rps15-ycf1_p5	TATACGACTCAGCTCCAGCA	GAACGACATGGAAGGATGCT	50.0	50.0	57.9	58.0	1442-1494 bp	1.0	84.5