Hotspot

rps8-rpl14

LSC · 82659-82830 · 172 bp

Back to Schisandra

Pi 0.0262

MarkerSeek score 75.50

Primer available yes

Label rps8-rpl14

Feature Metadata

label_name: rps8-rpl14
feature_type: igs
region: LSC
length_bp: 172
variable_sites: 10
pi: 0.0262
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 75.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps8-rpl14_p1	GGCAATAGTGTCCCTACCCA	GAACTCGAGTTTTTGGTGCG	55.0	50.0	58.8	58.0	273-384 bp	1.0	77.4
rps8-rpl14_p2	TGCTATTCGAACCGTTCCTT	GAACTCGAGTTTTTGGTGCG	45.0	50.0	57.2	58.0	333-444 bp	1.0	77.1
rps8-rpl14_p3	TCGGCAATAGTGTCCCTACC	GAACTCGAGTTTTTGGTGCG	55.0	50.0	58.9	58.0	275-386 bp	1.0	76.7
rps8-rpl14_p4	GGCAATAGTGTCCCTACCCA	ACTCGAGTTTTTGGTGCGAT	55.0	45.0	58.8	58.1	271-382 bp	1.0	76.6
rps8-rpl14_p5	TGCTATTCGAACCGTTCCTT	ACTCGAGTTTTTGGTGCGAT	45.0	45.0	57.2	58.1	331-442 bp	1.0	76.3