Hotspot

petB-petD

LSC · 78480-79332 · 853 bp

Back to Schisandra

Pi 0.00516

MarkerSeek score 63.40

Primer available yes

Label petB-petD

Feature Metadata

label_name: petB-petD
feature_type: igs
region: LSC
length_bp: 853
variable_sites: 9
pi: 0.00516
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 63.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petB-petD_p1	CAATCCACTTTGACCCGTTT	CTTAGAGATCCATCCCCCGA	45.0	55.0	56.5	57.7	960-1006 bp	1.0	70.5
petB-petD_p2	CAATCCACTTTGACCCGTTT	TAGAGATCCATCCCCCGAAG	45.0	55.0	56.5	57.7	958-1004 bp	1.0	70.5
petB-petD_p3	CAATCCACTTTGACCCGTTT	TCTTAGAGATCCATCCCCCG	45.0	55.0	56.5	57.7	961-1007 bp	1.0	70.5
petB-petD_p4	CAATCCACTTTGACCCGTTT	CCCATCCCTTTAGCCGATTT	45.0	50.0	56.5	57.6	1037-1083 bp	1.0	69.9
petB-petD_p5	CAATCCACTTTGACCCGTTTT	TAGAGATCCATCCCCCGAAG	42.9	55.0	57.3	57.7	958-1004 bp	1.0	68.4