Hotspot

trnT-TGT-trnF-GAA

LSC · 48756-50274 · 1519 bp

Back to Schisandra

Pi 0.0184

MarkerSeek score 69.90

Primer available yes

Label trnT-TGT-trnF-GAA

Feature Metadata

label_name: trnT-TGT-trnF-GAA
feature_type: igs
region: LSC
length_bp: 1519
variable_sites: 56
pi: 0.0184
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 69.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnT-TGT-trnF-GAA_p1	GCGATGCTCTAACCTCTGAG	TCTACCAGCTGAGCTATCCC	55.0	55.0	57.9	58.0	1422-1573 bp	1.0	85.8
trnT-TGT-trnF-GAA_p2	GCGATGCTCTAACCTCTGAG	ATTATCCACGTGCCAGGAAC	55.0	50.0	57.9	58.0	1482-1633 bp	1.0	85.4
trnT-TGT-trnF-GAA_p3	GCGATGCTCTAACCTCTGAG	TTTGAACTGGTGACACGAGG	55.0	50.0	57.9	58.1	1458-1609 bp	1.0	85.4
trnT-TGT-trnF-GAA_p4	GCGATGCTCTAACCTCTGAG	GTGCCAGGAACCAGATTTGA	55.0	50.0	57.9	58.1	1473-1624 bp	1.0	85.0
trnT-TGT-trnF-GAA_p5	GGAATCGAACCGATGACCAT	TCTACCAGCTGAGCTATCCC	50.0	55.0	57.8	58.0	1454-1605 bp	1.0	84.9