Hotspot

trnD-GTC-trnY-GTA

LSC · 32546-32957 · 412 bp

Back to Schisandra

Pi 0.0464

MarkerSeek score 82.90

Primer available yes

Label trnD-GTC-trnY-GTA

Feature Metadata

label_name: trnD-GTC-trnY-GTA
feature_type: igs
region: LSC
length_bp: 412
variable_sites: 39
pi: 0.0464
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 82.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnD-GTC-trnY-GTA_p1	CGGTGCTCTGACCAATTGAA	CTGGTTCAAATCCAGCTCGA	50.0	50.0	58.5	57.9	463-475 bp	1.0	84.5
trnD-GTC-trnY-GTA_p2	CGGTGCTCTGACCAATTGAA	TGGTTCAAATCCAGCTCGAC	50.0	50.0	58.5	58.2	462-474 bp	1.0	83.7
trnD-GTC-trnY-GTA_p3	CGGTGCTCTGACCAATTGAA	GGGGACGGACTGTAAATTCG	50.0	55.0	58.5	58.4	500-512 bp	1.0	81.8
trnD-GTC-trnY-GTA_p4	CGGTGCTCTGACCAATTGAA	GGGACGGACTGTAAATTCGT	50.0	50.0	58.5	57.6	499-511 bp	1.0	81.6
trnD-GTC-trnY-GTA_p5	CGGTGCTCTGACCAATTGAA	GGACTGTAAATTCGTTGGCG	50.0	50.0	58.5	57.5	494-506 bp	1.0	80.5