Hotspot

atpF-atpH

LSC · 14461-14962 · 502 bp

Back to Schisandra

Pi 0.0507

MarkerSeek score 78.60

Primer available yes

Label atpF-atpH

Feature Metadata

label_name: atpF-atpH
feature_type: igs
region: LSC
length_bp: 502
variable_sites: 53
pi: 0.0507
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 78.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpF-atpH_p1	GAAAGAATCGGTTACATTTTTCA	GCTTTTATTTGCGAATCCTTTTGT	30.4	33.3	53.9	57.5	538-576 bp	1.0	51.7
atpF-atpH_p2	AAAGAATCGGTTACATTTTTCA	GCTTTTATTTGCGAATCCTTTTGT	27.3	33.3	52.6	57.5	537-575 bp	1.0	51.7
atpF-atpH_p3	GAAAGAATCGGTTACATTTTTCAT	GCTTTTATTTGCGAATCCTTTTGT	29.2	33.3	54.5	57.5	538-576 bp	1.0	51.7
atpF-atpH_p4	AAGAATCGGTTACATTTTTCAT	GCTTTTATTTGCGAATCCTTTTGT	27.3	33.3	52.4	57.5	536-574 bp	1.0	51.7
atpF-atpH_p5	GAAAGAATCGGTTACATTTTTCA	GCTTTTATTTGCGAATCCTTTTG	30.4	34.8	53.9	56.0	538-576 bp	1.0	51.7