Hotspot

trnS-GCT-trnR-TCT

LSC · 9496-11386 · 1891 bp

Back to Schisandra

Pi 0.0265

MarkerSeek score 73.70

Primer available yes

Label trnS-GCT-trnR-TCT

Feature Metadata

label_name: trnS-GCT-trnR-TCT
feature_type: igs
region: LSC
length_bp: 1891
variable_sites: 98
pi: 0.0265
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 73.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-GCT-trnR-TCT_p1	CAATCCGACGCTTTAGTCCA	AGAAGACCTCTGTCCTATCCA	50.0	47.6	58.0	56.9	1417-1977 bp	1.0	71.8
trnS-GCT-trnR-TCT_p2	AATCCGACGCTTTAGTCCAC	AGAAGACCTCTGTCCTATCCA	50.0	47.6	58.0	56.9	1416-1976 bp	1.0	71.7
trnS-GCT-trnR-TCT_p3	CAATCCGACGCTTTAGTCCA	AGGTTTAGAAGACCTCTGTCCT	50.0	45.5	58.0	57.9	1423-1983 bp	1.0	71.1
trnS-GCT-trnR-TCT_p4	AATCCGACGCTTTAGTCCAC	AGGTTTAGAAGACCTCTGTCCT	50.0	45.5	58.0	57.9	1422-1982 bp	1.0	71.1
trnS-GCT-trnR-TCT_p5	CGGAGAGAGAGGGATTCGAA	AGAAGACCTCTGTCCTATCCA	55.0	47.6	58.3	56.9	1470-2030 bp	1.0	69.1